Antibodies are highly specific in their recognition of antigens. This ELISA experiment demonstrates the quantitation of varying concentrations of viral antigens as detected by the intensity of the colour reaction due to the accumulation of products.
- Master the Enzyme-Linked Immunosorbent Assay (ELISA) with this advanced experiment
- Prepare serial dilutions and perform ELISAs to determine the concentration of two antigens
- Explore the specificity of ELISAs by testing multiple antigen-antibody combinations
- Quantify results using imaging and graphing software
- Group size: for 10 lab groups
- Time required: complete in 2 hours
- Kit includes: instructions, antigens, primary; secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipettes
- All you need: distilled or deionised water, 37°C incubation oven, automatic micropipettes with tips, laboratory glassware.
- Storage: some components require refrigerator storage
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